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Agarose Gel Reagents and Buffers

Agarose is a highly purified, naturally-occurring polysaccharide. Agarose gels are prepared simply by heating the powdered agarose in buffer - normally 1 x TAE (tris acetate electrophoresis buffer) or 1 x TBE (tris borate electrophoresis buffer) - to dissolve it. It will then solidify to form a gel upon cooling. Like acrylamide, the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels, making them suitable for the separation of much larger nucleic acid fragments. There are many types of agarose available. The best choice for routine DNA electrophoresis is 'Agarose for DNA Electrophoresis' (Catalogue Number 11404), which offers a good gel strength and few impurities to interfere with subsequent molecular biological procedures, such as restriction digestion and ligation.


TBE Buffer (10x)


TBE buffer is widely used in molecular biology and nucleic acid electrophoresis and has a higher buffering capacity than TAE buffer. It can be used for DNA and RNA, polyacrylamide and agarose gel electrophoresis.
Supplied as a 10x concentrate....

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TAE Buffer (10x)


TAE buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffering capacity than TBE, although linear dsDNA tends to run faster in TAE than in TBE buffer.
Supplied as a 10x concentrate. (0.4M Tris, 0.2M Acetic Acid, 0.01M ED...

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DNA Marker

The Lambda x BstE II DNA marker contains 14 fragments ranging from 117 to 8,454 bp: 117, 224, 702, 1264, 137, 1929, 2323, 3675, 4324, 4822, 5687, 6369, 7242, and 8454 bp (the 5687 bp and 8453 bp fragments contain the cohesive ends of bacteriophage...

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Ethidium Bromide, Aqueous Solution, 1% w/v

Suitable for use in DNA isolation procedures and post-electrophoretic DNA staining. Concentration: 10mg/ml.

Related Products - Please click here for our full range of horizontal gel electrophoresis and casting products

 
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Ethidium Bromide Destaining Bags

Ethidium Bromide Destaining Bags
Each bag will remove up to 5mg of Ethidium Bromide from solution, from an overnight preparation. The rate of destaining is accelerated by adding more destaining bags to the solution.

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