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Isoelectric Focusing

Isoelectric Focusing (IEF) is an ingenious process for the simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (ampholytes) to resolve proteins according to their different pI (isoelectric point) values - i.e. the pH at which a protein has no net charge. IEF is an end-point method; when the electrophoresis run is completed proteins will appear as separate sharp zones, in the order of their pI. Scie-Plas is delighted to offer the following SERVA products for IEF, using immobilised pH gradient (IPG) strips, capillary ampholyte gels or precast IEF gels.


Ampholytes for Capillary Gel IEF


SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species with an average molecular weight distribution of 400 to 1000 Da, and comprise a multitude of var...

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SERVA Precast Gels for Isoelectric Focusing

With SERVALYT™ PRECOTES™ precast gels, the days of elaborate, inconvenient casting of gels are gone.  SERVA offers ready-to-use polyacrylamide gels for horizontal IEF that are easy to handle and reliable in performance.
The ...

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SERVALYTTM PRECOTESTM STARTER KIT

The SERVALYT™ PRECOTES™ Starter Kit contains three SERVALYT™ PRECOTES™ pH 3-10 standard precast gels (125 x 125 x 0.25mm), electrode wicks, applicator strips, anode and cathode solutions, Bayol F, SERVA Blue ...

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SERVA Liquid Mix IEF Marker pH 3-10

SERVA Liquid Mix IEF Marker pH 3-10 is a ready-to-use protein marker developed especially for IEF applications. The marker is applicable to all horizontal and vertical IEF gels and can be used in both native and denaturing conditions (e.g. contain...

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Applicator Strips


Four different silicone applicator strips for loading sample on to SERVALYT™ PRECOTES gels.




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Electrode Wicks

Available in three sizes: -


  • Standard - 120 x 6 x 1mm

  • Long - 240 x 6 x 1mm

  • Extra Long - 300 x 6 x 1mm


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    Electrode Fluid

    Anode Fluid, pH3 - 0.17g L-aspartic acid / 0.18g L-glutamic acid in water Cathode Fluid, pH10


    Cathode Fluid, pH10 - 0.22g L-arginine, 0.18g L-lysine, 6.0ml ethylenediamine in water.



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    Bayol F and Kerosene Pure

    Bayol F and Kerosene Pure maintain adequate heat dissipation, which helps to prevent desiccation of IEF gels and IPG strips during IEF.

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    SERVA IPG BlueStrips

    SERVA IPG BlueStrips are dried gel strips, incorporating an immobilised pH gradient, used in high resolution 2-D gel electrophoresis of proteins.  Ideal for use with the Scie-Plas dedicated 2-D systems and IEF-SYS unit.
    BENEFITS INCLUDE: -
    ...

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    Rehydration Tray

    To rehydrate up to 12 IPG strips of lengths up to 24cm. The rehydration tray maintains its shape and is resistant against the chemicals normally used to rehydrate IPG strips.



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    CONTACT US
    Scie-Plas Ltd.
    East Wing, Building 1020
    Cambourne Business Park
    Cambourne
    Cambridge, UK
    CB23 6DW

    Tel:
    +44 (0) 1223 427888
    Fax:
    +44 (0) 1223 420164

    E-mail:
    Sales@scie-plas.co.uk
    COPYRIGHT © SCIE-PLAS - MOST RECENT REVISION 3.7.2020