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Ampholytes for Capillary Gel IEF

SERVALYTM Carrier Ampholytes



SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species with an average molecular weight distribution of 400 to 1000 Da, and comprise a multitude of varying pI values. In agarose and polyacrylamide gels containing ampholytes, a linear pH gradient is generated when an electric field is applied. Each ampholyte molecule carries a net charge and will migrate within an electric field between the electrodes until it reaches the position corresponding to its pI, when it then stops moving to form a small plateau (stationary stack).


BENEFITS INCLUDE



  • High resolution due to multimeric composition

  • Fast staining and destaining times

  • Clear background associated with very low nonspecific binding of dyes and stains

  • High solubility in trichloroacetic acid (fast removal of ampholytes during fixation)

  • Virtually no interaction with metal ions


Please click the highlighted text for details of the properties and applications of SERVA capillary ampholytes.


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