For safe and easy protein staining

Coomassie Blue G250 is widely used to visualise proteins separated by agarose or acrylamide gel electrophoresis. This normally involves lengthy staining and destaining of gels using glacial acetic acid and methanol. These solvents are both toxic and poisonous and need to be disposed as hazardous waste.

In order to avoid using these materials, Coomassie Blue G250 has been formulated into a colloid (CCB), which is both non-toxic and non-hazardous. The colloidal solution presents a safer way to apply the dye to the gels and is both easy and less expensive to use.

The CCB stain is applied to the gel after washing the gel with purified deionised water. The CCB solution is then agitated until the bands start to appear within the gel, any time after 20 minutes up to two hours.

Once the bands appear and are of sufficient intensity, simply pour off the CCB stain and wash the gel in water. Keep washing until the background staining is removed and the protein bands appear more intense. This can be further enhanced by using CoZap™, a quick and easy destainer for Coomassie Blue.

Related products: -

• CoZap™ Destaining Pads


• Vertical gel electrophoresis units